Journal: Cell Proliferation
Article Title: Effects and mechanisms of basic fibroblast growth factor on the proliferation and regenerative profiles of cryopreserved dental pulp stem cells
doi: 10.1111/cpr.12969
Figure Lengend Snippet: Osteogenic and adipogenic differentiation of the succeeding passage from bFGF pre‐treated DPSCs. In the Control group, DPSCs (P5) were cultured in bFGF‐free medium and not induced. In the Induction‐CM group, cells (P5) were cultured without bFGF and induced. In the Induction‐prime 20 ng/mL bFGF group, cells (P5) were passaged from bFGF pre‐treated DPSCs and induced. In the Control, cells did not display newly regenerated structure (top panel in A and C). At low and high magnification of induction groups (middle and right column in A and C), mineralized nodules were stained by Alizarin Red and lipid droplets were stained by Oil Red O Scale bar : 200 μm and 100 μm. (B, D) Semi‐quantification of the newly regenerated structure. Induction groups sustained similar potential to differentiate into osteoblast or adipocyte. All data were represented as mean ± SD (n = 3), *** P < .001 vs the Control group
Article Snippet: In osteogenic and adipogenic differentiation, the OriCellTM MSCs osteogenic and adipogenic induction medium (Cyagen, Santa Clara, USA) were added to 90% confluent DPSCs (P5) according to the manufacturer's instructions.
Techniques: Control, Cell Culture, Staining